||FEI (now Thermo Fisher)
The Tecnai TF-30 is a transmission electron microscope (TEM) that can also be operated in scanning transmission electron microscopy (STEM) mode, with a voltage range of 80 to 300 kV. Operation at 300 kV makes this the KNI's highest resolution TEM (see also the 200 kV Tecnai TF-20). The TF-30 is also equipped with a high-angle annular dark field (HAADF) detector for use in STEM mode, and an energy dispersive spectroscopy (EDS) detector for compositional analysis (in both TEM mode and, most usually, in STEM mode). The Serial EM program allows for automated collection of images at variable tilt angles for performing tomography. See a full list of training and educational resources for this instrument below.
- High-Resolution TEM (HRTEM) imaging without an objective aperture
- Bright Field (BF) & Dark Field (DF) imaging with an objective aperture
- Selected Area Electron Diffraction (SAED)
- STEM imaging with a High-Angle Annular Dark Field (HAADF) detector
- Energy Dispersive Spectroscopy (EDS) with Oxford INCA system
- Automatically capture tilt series of images for tomographic imaging
SOPs & Troubleshooting
Order Your Own Stubs
- Stubs used for mounting specimens are considered a personal, consumable item in the KNI. There are some old stubs at each SEM, yet you should buy your own so that you can keep them clean and available to you. There are many stub geometries and configurations, some of which will be right for you to purchase and keep with your other cleanroom items.
- 0.5 to 30.0 kV
- Apertures: 10 mm, 15 mm, 20 mmm, 30 mm
- 5.0 to 30.0 kV
- 10 pA - 20 nA